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Recent studies have highlighted the up-regulation and necessity of one-carbon metabolism for tumor cell proliferation, thus making this metabolic pathway a viable therapeutic target (see Chandel 2020b)

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After 96 h, the cells were harvested using pancreatic enzymes, washed twice with PBS, and lysed with 1% TritonX-100 (300 μL for 0 μM CGA and 150 μL for 600 μM CGA) on ice for 10 min