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A western diet, but not a high-fat and low-sugar diet, predisposes mice to enhanced susceptibility to imiquimod-induced psoriasiform dermatitis

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(4) Centrifuge for 10 min (12000 rpm, 4°C), aspirate 150 μL of the supernatant with a syringe, filter it using a 0.22 μm organic phase pinhole filter, transfer it to an LC injection vial and store it at -80°C until LC-MS analysis