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For anterograde tracing of the PVN → DMV circuit, rAAV-hSyn-Cre-mCherry-WPRE-hGH polyA (AAV2/1-hSyn-Cre-mCherry, AAV2/1, 1.03 × 10 13 vg/mL) virus was injected into the PVN and Cre-dependent rAAV-EF1α-DIO-mCherry-WPRE-hGH pA (AAV-DIO-mCherry, AAV2/9, 5.31 × 10 12 vg/mL) virus was injected into the DMV of C57 mice

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Formulators can leverage these findings to create products that deliver visible firming and wrinkle-reducing benefits