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In a 2003 study by Canapp and colleagues published in Veterinary Surgery, topical GHK-Cu (Iamin 2% gel) applied daily to full-thickness ischemic wounds in 24 Sprague-Dawley rats reduced wound area by 64.5% at day 13, compared with 45.6% for vehicle and 28.2% for untreated controls, with significantly lower TNF-alpha and MMP-2/MMP-9 concentrations in treated wounds