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Determination of GPx (EC 1.11.1.9) activity GPx activity was measured spectrophotometrically (17) at 340 nm in 50 mM phosphate, 5 mM EDTA, pH 7.0 containing 0.3 mM NADPH, 0.3 U/mL GRx, 5 mM GSH, 4 mM sodium azide, 75 μM H 2 O 2 and 10 μL of erythrocyte lysate in a final reaction mixture of 3 mL