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4.1 Study limitations and future directions First, the brain weight of HI-treated animals may be higher than that of controls due to cerebral edema occurring during and after the HI injury ( Ultimately, the understanding of systemic and brain PK of 7,8-DHF among neonatal mice could be applied to larger animal models of neonatal HIE especially the ones that show the kidney and liver injury phenotype that is not present in Vannucci’s neonatal rodent model of HI