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Loading of Ran-His with GTPγS or GDP Purified Ran-His protein was loaded with GTPγS or GDP in the following reaction: 3.0 μM Ran-His was incubated with 0.5 mM GTPγS or GDP at 30 °C for 10 min in 20 mM Tris-HCl Ph 7.5, 100 mM NaCl, 5 mM EDTA, 2 mM DTT