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Tungsten particles coated with DiI (Molecular Probes, Life Technologies, Grand Island, NY, USA) were delivered into fixed coronal sections (150 μm thickness) using a Helios Gene Gun system (Bio-Rad Laboratories, Hercules, California, USA) and allowed to diffuse for 24–48 hr at 4 °C, followed by fixing in 4% PFA for 1 hr
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