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However, abnormal levels may delay the extrusion of the second polar body and result in low fertilization rates, as observed in mice (Buschiazzo et al

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Organoids were stimulated as described above for 24 h, washed with PBS and fixed with 4% PFA at room temperature for 20 min, washed again twice with PBS and permeabilized with PBS-Triton X 0.5% for 20 min at room temperature